Journal: BMC Research Notes

Article Title: Induction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants

doi: 10.1186/1756-0500-7-414

Figure Lengend Snippet: Capsicum annuum explants co-cultured with different Agrobacterium rhizogenes strains for one month. (a) Hypocotyl with ATCC 15834, (b) hypocotyl with ATCC 43056, (c) hypocotyl with ATCC 13333, (d) hypocotyl with ATCC 43057, (e) radicle with ATCC 15834, (f) radicle with ATCC 43056, (g) radicle with ATCC13333, (h) radicle with ATCC 43057, (i) cotyledon with ATCC 15834, (j) cotyledon with ATCC 43056, (k) cotyledon with ATCC13333 and (l) cotyledon with ATCC 43057.

Article Snippet: Three types of explants—cotyledon, hypocotyl, and radical—from each Capsicum species were co-cultured with four isolated A. rhizogenes wild type strains (ATCC 15834, ATCC 43056, ATCC 13333, and ATCC 43057).

Techniques: Cell Culture

Journal: BMC Research Notes

Article Title: Induction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants

doi: 10.1186/1756-0500-7-414

Figure Lengend Snippet: Capsicum frutescens explants co-cultured with different A. rhizogenes strains for 1 month. (a) Hypocotyl with ATCC 15834, (b) hypocotyl with ATCC 43056, (c) hypocotyl with ATCC 13333, (d) hypocotyl with ATCC 43057, (e) radicle with ATCC 15834, (f) radicle with ATCC 43056, (g) radicle with ATCC13333, (h) radicle with ATCC 43057, (i) cotyledon with ATCC 15834, (j) cotyledon with ATCC 43056, (k) cotyledon with ATCC13333 and (l) cotyledon with ATCC 43057.

Article Snippet: Three types of explants—cotyledon, hypocotyl, and radical—from each Capsicum species were co-cultured with four isolated A. rhizogenes wild type strains (ATCC 15834, ATCC 43056, ATCC 13333, and ATCC 43057).

Techniques: Cell Culture

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Effects of different Agrobacterium rhizogenes strains on hairy root growth of O. basilicum .

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques: Transformation Assay

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Dry weight of O. basilicum hairy root (g/30 ml medium) induced by different Agrobacterium rhizogenes strains. The data were obtained after 14 days of culturing in MS liquid medium. Data were obtained from three biological replicates. Different letters indicate the significant difference at p < 0.05 by Duncan’s multiple range tests.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Phenolic acid content in the induced hairy roots of O. basilicum by various Agrobacterium rhizogenes strains (µg/mg DW).

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Heatmap representing the differences in relative metabolite concentrations in O. basilicum hairy root induced by different Agrobacterium rhizogenes strains. Increases and decreases in the metabolites are shown in red and blue color, respectively.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Score and loading plot of the (A) PCA model and (B) PLS-DA results obtained from the metabolites identified in O. basilicum hairy root induced by different Agrobacterium rhizogenes strains.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Correlation matrix of metabolites identified in O. basilicum hairy root induced by different Agrobacterium rhizogenes strains. Each colored box indicates the Pearson’s correlation coefficient for a pair of compounds, and the value of the correlation coefficient is represented by the intensity of the blue or red color, as indicated on the color scale.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: Identified metabolites and their pathway impact on O. basilicum hairy root induced by different Agrobacterium rhizogenes strains. 1) Sphingolipid metabolism; 2) Valine, leucine, and isoleucine degradation; 3) Glycerophospholipid metabolism; 4) Selenocompound metabolism; 5) Phenylalanine metabolism; 6) Isoquinoline alkaloid biosynthesis; 7) Tryptophan metabolism; 8) Indole alkaloid biosynthesis; 9) Tropane, piperidine and pyridine alkaloid biosynthesis; 10) Nicotinate and nicotinamide metabolism; 11) Porphyrin and chlorophyll metabolism; 12) Fructose and mannose metabolism; 13) Propanoate metabolism; 14) Ascorbate and aldarate metabolism; 15) Terpenoid backbone biosynthesis; 16) Nitrogen metabolism; 17) Phosphatidylinositol signaling system; 18) Valine, leucine and isoleucine biosynthesis; 19) Cysteine and methionine metabolism; 20) Amino sugar and nucleotide sugar metabolism; 21) Glycolysis/Gluconeogenesis; 22) Sulfur metabolism; 23) Purine metabolism; 24) Lysine degradation; 25) Lysine biosynthesis; 26) Monobactam biosynthesis; 27) Glucosinolate biosynthesis; 28) Glycerolipid metabolism; 29) Phenylalanine, tyrosine and tryptophan biosynthesis; 30) Starch and sucrose metabolism; 31) Beta-alanine metabolism; 32) Pyruvate metabolism; 33) Arginine and proline metabolism; 34) Stilbenoid, diarylheptanoid and gingerol biosynthesis; 35) Glutathione metabolism; 36) Butanoate metabolism; 37) Galactose metabolism; 38) Arginine biosynthesis; 39) Inositol phosphate metabolism; 40) Pantothenate and CoA biosynthesis; 41) Pyrimidine metabolism; 42) Thiamine metabolism; 43) Pentose and glucoronate interconversions; 44) Flavonoid biosynthesis; 45) Carbon fixation in photosynthetic organisms; 46) Cyanomino acid metabolism; 47) Aminoacyl-tRNA biosynthesis; 48) Tyrosine metabolism; 49) Citrate cycle (TCA cycle); 50) Ubiquinone and other terpenoid-quinone biosynthesis; 51) Phenylpropanoid biosynthesis; 52) Glyoxylate and dicarboxylate metabolism; 53) Alanine, aspartate, and glutamate metabolism; 54) Glycine, serine, and threonine metabolism; 55) Biosynthesis of secondary metabolites-unclassified.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques: Starch

Journal: Frontiers in Plant Science

Article Title: Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum

doi: 10.3389/fpls.2022.983776

Figure Lengend Snippet: The key components, separating the O. basilicum hairy root induced by different Agrobacterium rhizogenes strains, are based on the VIP scores obtained through the PLS-DA model.

Article Snippet: To determine the transformation efficiency, six different strains of A. rhizogenes (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) were used for the experiment.

Techniques: